p21/CIP1/CDKN1A Antibody (4Z1H9)
Novus Biologicals | Catalog # NBP3-15661
Recombinant Monoclonal Antibody
![Western Blot: p21/CIP1/CDKN1A Antibody (4Z1H9) [NBP3-15661]](https://resources.rndsystems.com/images/products/p21-CIP1-CDKN1A-Antibody-1L4J1-Western-Blot-NBP3-15661-img0010.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (4Z1H9) [NBP3-15661]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Z1H9 expressed in HEK293
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human p21/CIP1/CDKN1A (NP_000380.1). MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p21/CIP1/CDKN1A Antibody (4Z1H9)
Western Blot: p21/CIP1/CDKN1A Antibody (4Z1H9) [NBP3-15661]
Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.
Analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.
Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -
Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] -
Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.Applications for p21/CIP1/CDKN1A Antibody (4Z1H9)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/m
Immunohistochemistry
1:200-1:800
Immunohistochemistry-Paraffin
1:200-1:800
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
Additional p21/CIP1/CDKN1A Products
Product Documents for p21/CIP1/CDKN1A Antibody (4Z1H9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for p21/CIP1/CDKN1A Antibody (4Z1H9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for p21/CIP1/CDKN1A Antibody (4Z1H9)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Apoptosis Signaling Pathway
