p21/CIP1/CDKN1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19652

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Chicken

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21/CIP1/CDKN1A. The exact sequence is proprietary.

Reactivity Notes

Cat (84%), Sheep (82%) Bovine (83%).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p21/CIP1/CDKN1A Antibody - BSA Free

p21/CIP1/CDKN1A Antibody

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody [NBP2-19652] -

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody [NBP2-19652] - p21/CIP1/CDKN1A antibody detects p21/CIP1/CDKN1A protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
p21/CIP1/CDKN1A stained by p21/CIP1/CDKN1A antibody (NBP2-19652) diluted at 1:500.
Antigen Retrieval: EDTA buffer, 20 min
p21/CIP1/CDKN1A Antibody - BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody [NBP2-19652] -

p21 antibody detects p21 protein by Western blot analysis.
A. 30 ug HCT116 whole cell extract (untreated)
B. 30 ug HCT116 whole cell extract (30 uM Cisplatin treatment for 24 h)
C. 30 ug HCT116 whole cell extract (30 uM Cisplatin treatment for 48 h)
12 % SDS-PAGE
p21 antibody (NBP2-19652) dilution: 1:1000
p21/CIP1/CDKN1A Antibody - BSA Free

Immunohistochemistry: p21/CIP1/CDKN1A Antibody [NBP2-19652] -

p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1xenograft.
p21 Cip1 antibody (NBP2-19652) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
p21/CIP1/CDKN1A Antibody - BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody [NBP2-19652] -

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (NBP2-19652) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for p21/CIP1/CDKN1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

UniProt

P38936

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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