P2X7/P2RX7 Antibody

Novus Biologicals | Catalog # NBP1-20180

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all P2X7/P2RX7 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse P2X7 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Marmoset (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P2X7/P2RX7 Antibody

Western Blot: P2X7/P2RX7 Antibody [NBP1-20180]

Western Blot: P2X7/P2RX7 Antibody [NBP1-20180]

Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin
Western Blot: P2X7/P2RX7 Antibody [NBP1-20180]

Western Blot: P2X7/P2RX7 Antibody [NBP1-20180]

Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin

Applications for P2X7/P2RX7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size (0.025ml) is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P2X7/P2RX7

FUNCTION: Receptor for ATP that acts as a ligand gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.; Subcellular location: Membrane; Multi-pass membrane protein.

Long Name

P2X Purinergic Receptor 7

Alternate Names

P2RX7

Entrez Gene IDs

5027 (Human); 18439 (Mouse); 29665 (Rat)

Gene Symbol

P2RX7

UniProt

Q9Z1M0 (Mouse)

Additional P2X7/P2RX7 Products

Product Documents for P2X7/P2RX7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for P2X7/P2RX7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for P2X7/P2RX7 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for P2X7/P2RX7 Antibody

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  • Q: I would like to know the size of product NBP1-20180. This is a lyophilized protein while the datasheet says it is 0.1ml. Could you please help explain?

    A: This antibody is supplied lyophilized as stated on the product page and datasheet. The antibody is from mouse whole antisera (0.1 ml of the sera in lyophilized form), so the concentration is unquantifiable. It cannot be sold as a mass amount since the concentration of the sera was not measured. We suggest to reconstitute it with sterilized water to 0.1 ml and then the  dilutions can be referenced for the particular application of choice from the datasheet.

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