P4HA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33878

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all P4HA2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P4HA2 Antibody - BSA Free

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Analysis in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.
Western Blot: P4HA2 Antibody [NBP2-33878]

Western Blot: P4HA2 Antibody [NBP2-33878]

Western Blot: P4HA2 Antibody [NBP2-33878] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: P4HA2 Antibody [NBP2-33878]

Immunocytochemistry/ Immunofluorescence: P4HA2 Antibody [NBP2-33878]

Immunocytochemistry/Immunofluorescence: P4HA2 Antibody [NBP2-33878] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human epididymis shows high expression.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878]

Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
P4HA2 Antibody Western Blot: P4HA2 Antibody Antibody [NBP2-33878]

Western Blot: P4HA2 Antibody Antibody [NBP2-33878]

Analysis using NBP2-33878 (A) shows similar pattern to independent antibody NBP2-33909 (B).

Applications for P4HA2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P4HA2

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

2-oxoglutarate-4-dioxygenase subunit alpha-2, 4-PH alpha 2, 4-PH alpha-2, alpha polypeptide II, collagen prolyl 4-hydroxylase alpha(II), C-P4Halpha(II), EC 1.14.11.2, Procollagen-proline, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), prolyl 4-hydroxylase subunit alpha-2, prolyl 4-hydroxylase, alpha polypeptide II

Gene Symbol

P4HA2

UniProt

O15460 (Human)

Additional P4HA2 Products

Product Documents for P4HA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for P4HA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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