p53 Antibody (rTP53/8993)

Name: p53 Antibody (rTP53/8993)
Brand: Novus Biologicals
SKU: NBP3-23711
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23711

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ kappa Clone # rTP53/8993

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Product Specifications

Immunogen

Recombinant human full-length p53 protein

Localization

Nucleus.

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for p53 Antibody (rTP53/8993)

Immunohistochemistry-Paraffin: p53 Antibody (rTP53/8993) [NBP3-23711] -

Immunohistochemistry-Paraffin: p53 Antibody (rTP53/8993) [NBP3-23711] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with p53 Antibody (rTP53/8993). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for p53 Antibody (rTP53/8993)

Application

Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml

Application Notes

Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

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Product Documents for p53 Antibody (rTP53/8993)

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Certificate of Analysis

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Product Specific Notices for p53 Antibody (rTP53/8993)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.