p97/VCP Antibody (4A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00007415-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A8

Format

Azide and BSA Free
View all VCP Primary Antibodies »

Product Specifications

Immunogen

VCP (AAH07562, 1 a.a. ~ 588 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

VCP - valosin-containing protein (4A8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p97/VCP Antibody (4A8) - Azide and BSA Free

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.
Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03]

Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03]

Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ).
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in PC-12.
Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03]

Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Raw 264.7.
ELISA: p97/VCP Antibody (4A8) [H00007415-M03]

ELISA: p97/VCP Antibody (4A8) [H00007415-M03]

ELISA: p97/VCP Antibody (4A8) [H00007415-M03] - Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.

Applications for p97/VCP Antibody (4A8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VCP

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

7415 (Human)

Gene Symbol

VCP

UniProt

AAH07562 (Human)

Additional VCP Products

Product Documents for p97/VCP Antibody (4A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p97/VCP Antibody (4A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
NFkB Pathway

Citations for p97/VCP Antibody (4A8) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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