p97/VCP Antibody

Novus Biologicals | Catalog # NB100-1558

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VCP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 806) of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30010465). Mouse, Rat reactivity reported in scientific literature (PMID: 20833645).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p97/VCP Antibody

Western Blot: p97/VCP Antibody [NB100-1558]

Western Blot: p97/VCP Antibody [NB100-1558]

p97-VCP-Antibody-Western-Blot-NB100-1558-img0015.jpg
Western Blot: p97/VCP Antibody [NB100-1558]

Western Blot: p97/VCP Antibody [NB100-1558]

Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558]

Western Blot: p97/VCP Antibody [NB100-1558]

Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
p97/VCP Antibody

Western Blot: p97/VCP Antibody [NB100-1558] -

Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20 ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells & asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated & subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue & afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22607136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for p97/VCP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg of lysate

Western Blot

1:2000 - 1:10000
Application Notes
ICC/IF reactivity reported in (PMID: 23297223), IHC-Frozen reactivity reported in (PMID: 20833645).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCP

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

7415 (Human); 67459 (Mouse)

Gene Symbol

VCP

UniProt

P55072 (Human)

Additional VCP Products

Product Documents for p97/VCP Antibody

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
NFkB Pathway

Citations for p97/VCP Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for p97/VCP Antibody

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  • Q: I used the p97/VCP antibody (catalog # NB100-1558) and would now like to use for ICC. Is there a recommended dilution? In the Data Sheet can be found on the ICC not specified.

    A: For the product NB100-1558, p97/VCP Antibody, the recommended starting dilution for ICC/IF is 1:100 to 1:500. Please note that we always advise customers to investigate the optimal dilution for them, as this will depend on the particular samples/reagents/protocols being used.

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