PABP Antibody (10E10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80901

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine, Canine, Drosophila (Negative), Mouse (Negative), Primate, Xenopus

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10E10

Format

Azide and BSA Free
View all PABP Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PABP protein. [Uniprot# P11940]

Reactivity Notes

Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Localization

Cytoplasmic. Shuttles between the cytoplasm and the nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PABP Antibody (10E10) - Azide and BSA Free

Western Blot: PABP Antibody (10E10)Azide and BSA Free [NBP2-80901]

Western Blot: PABP Antibody (10E10)Azide and BSA Free [NBP2-80901]

Western Blot: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901]

Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901]

Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.
Flow Cytometry: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901]

Flow Cytometry: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901]

Flow Cytometry: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Western Blot: PABP Antibody (10E10)Azide and BSA Free [NBP2-80901]

Western Blot: PABP Antibody (10E10)Azide and BSA Free [NBP2-80901]

Western Blot: PABP Antibody (10E10) - Azide and BSA Free [NBP2-80901] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates. Image from the standard format of this antibody.

Applications for PABP Antibody (10E10) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PABP

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

Alternate Names

PAB1polyadenylate-binding protein 1, PABP, PABP-1, PABP1poly(A) binding protein, cytoplasmic 2, PABPC2, PABPL1, poly(A) binding protein, cytoplasmic 1, Poly(A)-binding protein 1, poly(A)-binding protein, cytoplasmic 2

Gene Symbol

PABPC1

Additional PABP Products

Product Documents for PABP Antibody (10E10) - Azide and BSA Free

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Product Specific Notices for PABP Antibody (10E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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