PABP Antibody (10E10) - BSA Free

Novus Biologicals | Catalog # NB120-6125

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine, Canine, Drosophila (Negative), Mouse (Negative), Primate, Xenopus

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, CyTOF-ready

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10E10

Format

BSA Free
View all PABP Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PABP protein. [Uniprot# P11940]

Reactivity Notes

Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Localization

Cytoplasmic. Shuttles between the cytoplasm and the nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PABP Antibody (10E10) - BSA Free

Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - BSA Free [NB120-6125]

Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) - BSA Free [NB120-6125]

Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
Flow Cytometry: PABP Antibody (10E10) - BSA Free [NB120-6125]

Flow Cytometry: PABP Antibody (10E10) - BSA Free [NB120-6125]

Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10)BSA Free [NB120-6125]

Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.
PABP Antibody (10E10) - BSA Free

Western Blot: PABP Antibody (10E10) - BSA Free [NB120-6125] -

PI3K/AKT signaling contributes to HG-induced PABP expression, which promotes DENV infection.(A) Western blot showed the expression of PABP, NF90, hnRNP, eEF1A, PTB, YB-1, and beta -actin in 5.5 or 25 mM glucose (Glu) medium–treated BHK-21 cells for 48 hours. (B) Furthermore, the time course expression of PABP protein also is shown. (C) Western blot showed PABP protein expression in BHK-21 cells that were pretreated with or without PI3K inhibitor (LY294002), the mTOR inhibitor rapamycin (Rapa), or AKT inhibitor (AKTi) for 1 hour followed by 5.5 or 25 mM Glu-containing–medium treatment for 48 hours. (D) Real-time qPCR assays showed the expression of PABP mRNA in 5.5 or 25 mM Glu-treated BHK-21 cells that were pretreated with or without LY294002 and an AKTi for 1 hour and subsequently maintained in medium containing 5.5 or 25 mM Glu for 48 hours. (E) Western blot showed PABP protein expression in BHK-21 cells pretreated with PABP siRNA (siPABP) for 48 hours, followed by incubation with medium containing 25 mM Glu. Cells without control siRNA pretreatment were used as negative control. (F) Plaque assays were conducted to determine the viral titer of BHK-21 cells that were pretreated with PABP siRNA for 48 hours and then infected with DENV 2 (MOI, 1) for an additional 48 hours in 5.5 or 25 mM Glu-containing medium. DMSO was used as a control. The mean +/- SD of quantitative data from at least 3 independent experiments are reported. **P < 0.01, ***P < 0.001. RQ, relative quantification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36125898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PABP Antibody (10E10) - BSA Free

Western Blot: PABP Antibody (10E10) - BSA Free [NB120-6125] -

PI3K/AKT signaling contributes to HG-induced PABP expression, which promotes DENV infection.(A) Western blot showed the expression of PABP, NF90, hnRNP, eEF1A, PTB, YB-1, and beta -actin in 5.5 or 25 mM glucose (Glu) medium–treated BHK-21 cells for 48 hours. (B) Furthermore, the time course expression of PABP protein also is shown. (C) Western blot showed PABP protein expression in BHK-21 cells that were pretreated with or without PI3K inhibitor (LY294002), the mTOR inhibitor rapamycin (Rapa), or AKT inhibitor (AKTi) for 1 hour followed by 5.5 or 25 mM Glu-containing–medium treatment for 48 hours. (D) Real-time qPCR assays showed the expression of PABP mRNA in 5.5 or 25 mM Glu-treated BHK-21 cells that were pretreated with or without LY294002 and an AKTi for 1 hour and subsequently maintained in medium containing 5.5 or 25 mM Glu for 48 hours. (E) Western blot showed PABP protein expression in BHK-21 cells pretreated with PABP siRNA (siPABP) for 48 hours, followed by incubation with medium containing 25 mM Glu. Cells without control siRNA pretreatment were used as negative control. (F) Plaque assays were conducted to determine the viral titer of BHK-21 cells that were pretreated with PABP siRNA for 48 hours and then infected with DENV 2 (MOI, 1) for an additional 48 hours in 5.5 or 25 mM Glu-containing medium. DMSO was used as a control. The mean +/- SD of quantitative data from at least 3 independent experiments are reported. **P < 0.01, ***P < 0.001. RQ, relative quantification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36125898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PABP Antibody (10E10) - BSA Free

Western Blot: PABP Antibody (10E10) - BSA Free [NB120-6125] -

PI3K/AKT signaling contributes to HG-induced PABP expression, which promotes DENV infection.(A) Western blot showed the expression of PABP, NF90, hnRNP, eEF1A, PTB, YB-1, and beta -actin in 5.5 or 25 mM glucose (Glu) medium–treated BHK-21 cells for 48 hours. (B) Furthermore, the time course expression of PABP protein also is shown. (C) Western blot showed PABP protein expression in BHK-21 cells that were pretreated with or without PI3K inhibitor (LY294002), the mTOR inhibitor rapamycin (Rapa), or AKT inhibitor (AKTi) for 1 hour followed by 5.5 or 25 mM Glu-containing–medium treatment for 48 hours. (D) Real-time qPCR assays showed the expression of PABP mRNA in 5.5 or 25 mM Glu-treated BHK-21 cells that were pretreated with or without LY294002 and an AKTi for 1 hour and subsequently maintained in medium containing 5.5 or 25 mM Glu for 48 hours. (E) Western blot showed PABP protein expression in BHK-21 cells pretreated with PABP siRNA (siPABP) for 48 hours, followed by incubation with medium containing 25 mM Glu. Cells without control siRNA pretreatment were used as negative control. (F) Plaque assays were conducted to determine the viral titer of BHK-21 cells that were pretreated with PABP siRNA for 48 hours and then infected with DENV 2 (MOI, 1) for an additional 48 hours in 5.5 or 25 mM Glu-containing medium. DMSO was used as a control. The mean +/- SD of quantitative data from at least 3 independent experiments are reported. **P < 0.01, ***P < 0.001. RQ, relative quantification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36125898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PABP Antibody (10E10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NB120-6125 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PABP

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

Alternate Names

PAB1polyadenylate-binding protein 1, PABP, PABP-1, PABP1poly(A) binding protein, cytoplasmic 2, PABPC2, PABPL1, poly(A) binding protein, cytoplasmic 1, Poly(A)-binding protein 1, poly(A)-binding protein, cytoplasmic 2

Entrez Gene IDs

26986 (Human)

Gene Symbol

PABPC1

UniProt

P11940 (Human)

Additional PABP Products

Product Documents for PABP Antibody (10E10) - BSA Free

Certificate of Analysis

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Product Specific Notices for PABP Antibody (10E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PABP Antibody (10E10) - BSA Free

Customer Reviews for PABP Antibody (10E10) - BSA Free (1)

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  • PABP Antibody (10E10)
    Name: Stephanie DeWitte-Orr
    Application: Western Blot
    Sample Tested: RTG-2 cells total lysate and HFF-1 cells total lysate
    Species: Rainbow trout, Oncorhynchus mykiss and Human
    Verified Customer | Posted 05/02/2017
    20ug of total cell lysate (RIPA) from RTG-2 and HFF-1 cells. Did not show any signal for rainbow trout cells.
    PABP Antibody (10E10) - BSA Free NB120-6125

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