PABPN1 Antibody

Novus Biologicals | Catalog # NBP1-31805

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PABPN1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PABPN1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (88%).

Localization

Nucleus/Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PABPN1 Antibody (NBP1-31805) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PABPN1 Antibody

Western Blot: PABPN1 Antibody [NBP1-31805]

Western Blot: PABPN1 Antibody [NBP1-31805]

Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody [NBP1-31805]

Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody [NBP1-31805]

Immunocytochemistry/Immunofluorescence: PABPN1 Antibody [NBP1-31805] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABPN1 protein stained by PABPN1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805]

Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805]

Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805] - Human colon carcinoma, using PABPN1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PABPN1 Antibody [NBP1-31805]

Western Blot: PABPN1 Antibody [NBP1-31805]

Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Applications for PABPN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-FR- Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PABPN1

The PABPN1 gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the pol

Alternate Names

Nuclear poly(A)-binding protein 1, OPMD, PAB2poly(A) binding protein II, PABII, PABP-2, PABP2polyadenylate-binding protein 2, poly(A) binding protein 2, poly(A) binding protein, nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, poly(A)-binding protein, nuclear 1, Polyadenylate-binding nuclear protein 1

Gene Symbol

PABPN1

Additional PABPN1 Products

Product Documents for PABPN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PABPN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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