PACT Antibody (PSH02-15)

Western Blot: PACT Antibody (PSH02-15) [NBP3-32706]

Western Blot: PACT Antibody (PSH02-15) [NBP3-32706] - Western blot analysis of PACT on different lysates with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: K-562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: Hela cell lysate
Lane 7: HL-60 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse testis tissue lysate
Lane 11: Mouse liver tissue lysate
Lane 12: Rat testis tissue lysate
Lane 13: Rat brain tissue lysate

Cell lysates: 20ug/lane, tissue lysates: 40ug/lane

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 1 minutes 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32706) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706]

Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunofluorescence analysis of paraffin-embedded rat cerebral cortex tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32706, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706]

Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32706) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.