Pain Antibody

Novus Biologicals | Catalog # NB100-98737

Novus Biologicals
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Key Product Details

Species Reactivity

Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Pain Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from 3rd extracellular loop of drosophila Pain conjugated to an immunogenic carrier protein was used as the immunogen.

Specificity

Pain (Transient receptor potential cation channel protein painless).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Pain Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Frozen

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000
Application Notes
Useful in Immunofluorescence (PMID: 22073144).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pain

FUNCTION: Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. DEVELOPMENTAL STAGE: Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons.

Alternate Names

Painless protein, Transient protein painless, Transient receptor potential cation channel protein painless

Additional Pain Products

Product Documents for Pain Antibody

Certificate of Analysis

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Product Specific Notices for Pain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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