PAK1 Antibody (4D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005058-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D1

Format

Azide and BSA Free
View all PAK1 Primary Antibodies »

Product Specifications

Immunogen

PAK1 (NP_002567, 191 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. APRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGA

Localization

Cytoplasm. Recruited to focal adhesions upon activation.

Specificity

PAK1 - p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PAK1 Antibody (4D1) - Azide and BSA Free

Western Blot: PAK1 Antibody (4D1) [H00005058-M02]

Western Blot: PAK1 Antibody (4D1) [H00005058-M02]

Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02]

Immunocytochemistry/ Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02]

Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.
Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02]

Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02]

Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: PAK1 Antibody (4D1) [H00005058-M02]

Western Blot: PAK1 Antibody (4D1) [H00005058-M02]

Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).
Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02]

Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02]

Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.
Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.

Applications for PAK1 Antibody (4D1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAK1

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Entrez Gene IDs

5058 (Human)

Gene Symbol

PAK1

OMIM

602590 (Human)

UniProt

NP_002567 (Human)

Additional PAK1 Products

Product Documents for PAK1 Antibody (4D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PAK1 Antibody (4D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

Citations for PAK1 Antibody (4D1) - Azide and BSA Free

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Protocols

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FAQs

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