![Western Blot: PAK2 Antibody (3B5)BSA Free [NBP1-47542]](https://beta-resources.rndsystems.com/images/products/PAK2-Antibody-3B5-Western-Blot-NBP1-47542-img0006.jpg "Western Blot: PAK2 Antibody (3B5)BSA Free [NBP1-47542]")
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Key Product Details
Species Reactivity
Human, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3B5
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Product Specifications
Immunogen
Purified recombinant fragment of PAK2 expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse PAK2 Antibody (3B5) (NBP1-47542) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PAK2 Antibody (3B5)
Western Blot: PAK2 Antibody (3B5)BSA Free [NBP1-47542]
Western Blot: PAK2 Antibody (3B5) [NBP1-47542] - Western blot analysis using anti-PAK2 monoclonal antibody against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.![Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]](https://beta-resources.rndsystems.com/images/products/PAK2-Antibody-3B5-Immunocytochemistry-Immunofluorescence-NBP1-47542-img0007.jpg "Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]")
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]
Immunocytochemistry/Immunofluorescence: PAK2 Antibody (3B5) [NBP1-47542] - Confocal immunofluorescence analysis of Hela cells using anti-PAK2 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.![Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]](https://beta-resources.rndsystems.com/images/products/PAK2-Antibody-3B5-Immunohistochemistry-Paraffin-NBP1-47542-img0005.jpg "Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]")
Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) - BSA Free [NBP1-47542]
Immunohistochemistry-Paraffin: PAK2 Antibody (3B5) [NBP1-47542] - Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using anti-PAK2 antibody with DAB staining.Applications for PAK2 Antibody (3B5)
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1: 500-1:2000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAK2
Long Name
p21-activated Kinase 2
Alternate Names
gamma-PAK, PAK65, S6/H4 Kinase
Gene Symbol
PAK2
Additional PAK2 Products
Product Documents for PAK2 Antibody (3B5)
Product Specific Notices for PAK2 Antibody (3B5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
