pan Actin Antibody (AC-40)

Novus Biologicals | Catalog # NB120-11003

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Bovine, Canine, Chicken, Chinese Hamster, Goat, Guinea Pig, Primate, Rabbit, Reptile, Sheep

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # AC-40
View all pan Actin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: SGPSIVHALCP (human) attached to Multiple Antigen Peptide (MAP) backbone.

Epitope

Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

The does not react with the epitope on the N-terminal end of actin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for pan Actin Antibody (AC-40)

Western Blot: pan Actin Antibody (AC-40) [NB120-11003]

Western Blot: pan Actin Antibody (AC-40) [NB120-11003]

Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.
Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin
Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003]

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Applications for pan Actin Antibody (AC-40)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:500
Application Notes
For IHC: The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solutions may also be used as fixatives.
See Simple Western Antibody Database for Simple Western validation: tested in HEK293 and mouse brain; separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:200; apparent MW in kDa on Simple Western was 50 kDa; matrix was 12-230 kDa.

Reviewed Applications

Read 1 review rated 5 using NB120-11003 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: pan Actin

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, Alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Entrez Gene IDs

60 (Human); 71 (Human); 58 (Human); 11465 (Mouse); 287876 (Rat)

Gene Symbol

ACTB

Additional pan Actin Products

Product Documents for pan Actin Antibody (AC-40)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for pan Actin Antibody (AC-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for pan Actin Antibody (AC-40)

Customer Reviews for pan Actin Antibody (AC-40) (1)

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  • Name: Ranveer Singh
    Application: Western Blot
    Sample Tested: siRNA transfected whole cell lysate from siRNA transfected HEK 293T cells
    Species: Human
    Verified Customer | Posted 05/22/2014
    Actin western blot as loading control for siRNA transfections in Hek293T cells
    pan Actin Antibody (AC-40) NB120-11003

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Protocols

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