pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25605
Recombinant Monoclonal Antibody
![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940840.jpg "Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Drosophila
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1279 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr410 of human pan PKC zeta. The exact sequence is proprietary.
Modification
p Thr410
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024941594.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249342742.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249422634.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249415946.jpg "Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940812.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249383155.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: pan PKC
Alternate Names
AAG6;PKCA;PKCalpha;PKC-alpha;PKCI+/-;PRKACA;PRKCA;Protein kinase C;Protein kinase C alpha type
Gene Symbol
PRKCA
Additional pan PKC Products
Product Documents for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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