Parkin Antibody (1H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005071-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1H4

Format

Azide and BSA Free
View all Parkin Primary Antibodies »

Product Specifications

Immunogen

PARK2 (AAH22014, 288 a.a. ~ 387 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PCVGTGDTVVLRGALGGFRRGVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGYGQRRTK

Specificity

PARK2 - Parkinson disease (autosomal recessive, juvenile) 2, parkin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Parkin Antibody (1H4) - Azide and BSA Free

Western Blot: Parkin Antibody (1H4) [H00005071-M01]

Western Blot: Parkin Antibody (1H4) [H00005071-M01]

Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Parkin monoclonal antibody (M01), clone 1H4. Western Blot analysis of Parkin expression in PC-12.
Immunocytochemistry/ Immunofluorescence: Parkin Antibody (1H4) [H00005071-M01]

Immunocytochemistry/ Immunofluorescence: Parkin Antibody (1H4) [H00005071-M01]

Immunocytochemistry/Immunofluorescence: Parkin Antibody (1H4) [H00005071-M01] - Analysis of monoclonal antibody to PARK2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Parkin Antibody (1H4) [H00005071-M01]

Western Blot: Parkin Antibody (1H4) [H00005071-M01]

Western Blot: Parkin Antibody (1H4) [H00005071-M01] - PARK2 monoclonal antibody (M01), clone 1H4 Analysis of PARK2 expression in Jurkat.
ELISA: Parkin Antibody (1H4) [H00005071-M01]

ELISA: Parkin Antibody (1H4) [H00005071-M01]

ELISA: Parkin Antibody (1H4) [H00005071-M01] - Detection limit for recombinant GST tagged PARK2 is 0.1 ng/ml as a capture antibody.

Applications for Parkin Antibody (1H4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reviewed Applications

Read 2 reviews rated 3.5 using H00005071-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Parkin

The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Entrez Gene IDs

5071 (Human)

Gene Symbol

PRKN

OMIM

211980 (Human)

UniProt

AAH22014 (Human)

Additional Parkin Products

Product Documents for Parkin Antibody (1H4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Parkin Antibody (1H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Parkin Antibody (1H4) - Azide and BSA Free

Customer Reviews for Parkin Antibody (1H4) - Azide and BSA Free (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
50%
2 Stars
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1 Stars
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Customer Images

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  • Parkin Antibody (1H4)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse skeletal muscle homogenate
    Species: Mouse
    Verified Customer | Posted 09/26/2017
    Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Western blot analysisi was performed in mouse skeletal muscle proteins, separated by 4-12% SDS-PAGE. Proteins were transferred on nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. Anti Parkin antibody was used 1:1000
    Parkin Antibody (1H4) - Azide and BSA Free H00005071-M01
  • Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A cells (different total protein concentrations of the mitochondria-enriched fraction versus whole-cell lysate)
    Species: Human
    Verified Customer | Posted 08/22/2016
    PARK2 antibody on Simple Western using human SVG-A cells
    Parkin Antibody (1H4) - Azide and BSA Free H00005071-M01

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Protocols

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FAQs

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