PARP Antibody (194C1439) - Cleaved - BSA Free

Novus Biologicals | Catalog # NB100-56599

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 194C1439

Format

BSA Free
View all PARP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29622764). Rat negative reported via customer review.

Specificity

This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PARP Antibody (194C1439) - Cleaved - BSA Free

Western Blot: PARP Antibody (194C1439)CleavedBSA Free [NB100-56599]

Western Blot: PARP Antibody (194C1439)CleavedBSA Free [NB100-56599]

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.
Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - BSA Free [NB100-56599]

Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved - BSA Free [NB100-56599]

Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PARP Antibody (194C1439)CleavedBSA Free [NB100-56599]

Western Blot: PARP Antibody (194C1439)CleavedBSA Free [NB100-56599]

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.

Applications for PARP Antibody (194C1439) - Cleaved - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1 - 10 ug/1x10^6 cells

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Western Blot

1 - 2 ug/mL

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARP

The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL, cleaved PARP, cleaved PARP 1, cleaved PARP apoptosis, cleaved PARP western blot, cleaved PARP-1

Gene Symbol

PARP1

Additional PARP Products

Product Documents for PARP Antibody (194C1439) - Cleaved - BSA Free

Certificate of Analysis

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Product Specific Notices for PARP Antibody (194C1439) - Cleaved - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PARP Antibody (194C1439) - Cleaved - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PARP Antibody (194C1439) - Cleaved - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

  • Q: I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).

    A: For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.

  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

  • Q: I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).

    A: For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.

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