PARP/Apoptosis Colorimetric Assay Kit

For the measurment of PARP-1 activity in cell extracts
Catalog # Availability Size / Price Qty
4684-096-K
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PARP/Apoptosis Colorimetric Assay Kit Summary

Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Colorimetric readout
• 96 well format
• Highly sensitive – detects 0.1 mU PARP ~500 cells
• Dynamic range between 0.1 to 10 mU PARP
• Requires 10-100 ng extract for detection
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.  During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

Product Specifications

  • Measure PARP-1 activity in cell extracts before and after apoptosis.
  • For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 10X Activated DNA
• PARP-HSA, 10 mUnits/µl
• 20 mM NAD
• Anti-PAR monoclonal Antibody
• Goat anti-mouse IgG-HRP
• 10 mM Etoposide
• 20X I-PAR Assay Buffer
• 5X Antibody Diluent
• Histone-Coated Natural Strip Well Plate, I-PAR
• TACS-Sapphire

Kit Contents

  • 10X Activated DNA
  • PARP-HSA, 10 mUnits/µl
  • 20 mM NAD
  • Anti-PAR monoclonal Antibody
  • Goat anti-mouse IgG-HRP
  • 10 mM Etoposide
  • 20X I-PAR Assay Buffer
  • 5X Antibody Diluent
  • Histone-Coated Natural Strip Well Plate, I-PAR
  • TACS-Sapphire

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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