PARP/Apoptosis Universal Colorimetric Assay Kit

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4677-096-K
Inhibition of PARP Activity by 3-aminobenzamide_4677-096-K
1 Image
Product Details
FAQs
Reviews

PARP/Apoptosis Universal Colorimetric Assay Kit Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Colorimetric readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• PARP-HSA, 10 Units/µl
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• Histone-Coated Plate Natural Strip Well Plate
• Strep-HRP
• TACS-Sapphire

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Inhibition of PARP Activity by 3-aminobenzamide. Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.

Reagents Provided

FAQs

No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for PARP/Apoptosis Universal Colorimetric Assay Kit

There are currently no reviews for this product. Be the first to review PARP/Apoptosis Universal Colorimetric Assay Kit and earn rewards!

Have you used PARP/Apoptosis Universal Colorimetric Assay Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review