PARP/Apoptosis Universal Colorimetric Assay Kit
PARP/Apoptosis Universal Colorimetric Assay Kit Summary
• Colorimetric readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs
Why Use the PARP/Apoptosis Colorimetric Assay Kit?
This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
• PARP-HSA, 10 Units/µl
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• Histone-Coated Plate Natural Strip Well Plate
For research use only. Not for diagnositic use.
Inhibition of PARP Activity by 3-aminobenzamide. Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.
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