PARP/Apoptosis Universal Colorimetric Assay Kit

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4677-096-K
Inhibition of PARP Activity by 3-aminobenzamide_4677-096-K
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Product Details
Citations (23)
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PARP/Apoptosis Universal Colorimetric Assay Kit Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Colorimetric readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• PARP-HSA, 10 Units/µl
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• Histone-Coated Plate Natural Strip Well Plate
• Strep-HRP
• TACS-Sapphire

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Inhibition of PARP Activity by 3-aminobenzamide. Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.

Citations for PARP/Apoptosis Universal Colorimetric Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Inhibition of NADPH oxidase alleviates germ cell apoptosis and ER stress during testicular ischemia reperfusion injury
    Authors: F Al-Saleh, F Khashab, F Fadel, N Al-Kandari, M Al-Maghreb
    Saudi J Biol Sci, 2020;27(8):2174-2184.  2020
  2. Riluzole protects against skeletal muscle ischaemia-reperfusion injury in a porcine model
    Authors: RW Li, Y Deng, HN Pham, S Weiss, M Chen, PN Smith
    Injury, 2019;0(0):.  2019
  3. Increased error-free DNA repair gene expression through reprogramming in human iPS cells
    Authors: Y Yoshimura
    Regen Ther, 2019;11(0):101-105.  2019
  4. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2
    Authors: C Wang, W Xu, J An, M Liang, Y Li, F Zhang, Q Tong, K Huang
    Nat Commun, 2019;10(1):1203.  2019
  5. The Effect of Green and Black Tea Polyphenols on BRCA2 Deficient Chinese Hamster Cells by Synthetic Lethality through PARP Inhibition
    Authors: S Alqahtani, K Welton, JP Gius, S Elmegerhi, TA Kato
    Int J Mol Sci, 2019;20(6):.  2019
  6. TRAIL induces necroptosis involving RIPK1/RIPK3-dependent PARP-1 activation.
    Authors: Jouan-Lanhouet S, Arshad M, Piquet-Pellorce C, Martin-Chouly C, Le Moigne-Muller G, Van Herreweghe F, Takahashi N, Sergent O, Lagadic-Gossmann D, Vandenabeele P, Samson M, Dimanche-Boitrel M
    Cell Death Differ, 2012;19(12):2003-14.  2012
  7. PARP-1 inhibition prevents oxidative and nitrosative stress-induced endothelial cell death via transactivation of the VEGF receptor 2.
    Authors: Mathews MT, Berk BC
    Arterioscler. Thromb. Vasc. Biol., 2008;28(4):711-7.  2008
  8. Histone H2AX is a critical factor for cellular protection against DNA alkylating agents.
    Authors: Meador JA, Zhao M, Su Y, Narayan G, Geard CR, Balajee AS
    Oncogene, 2008;27(43):5662-71.  2008
  9. Differential effects of poly(ADP-ribose) polymerase inhibition on DNA break repair in human cells are revealed with Epstein-Barr virus.
    Authors: Ma W, Halweg C, Menendez D, Resnick M
    Proc Natl Acad Sci U S A, 0;109(17):6590-5.  0
  10. Silencing of poly(ADP-ribose) polymerase-1 suppresses hyperstretch-induced expression of inflammatory cytokines in vitro.
    Authors: Wang J, Liu L, Xia Y, Wu D
    Acta Biochim Biophys Sin (Shanghai), 0;46(7):556-64.  0
  11. BCL2 suppresses PARP1 function and nonapoptotic cell death.
    Authors: Dutta C, Day T, Kopp N, van Bodegom D, Davids M, Ryan J, Bird L, Kommajosyula N, Weigert O, Yoda A, Fung H, Brown J, Shapiro G, Letai A, Weinstock D
    Cancer Res, 0;72(16):4193-203.  0
  12. PARP inhibition ameliorates nephropathy in an animal model of type 2 diabetes: focus on oxidative stress, inflammation, and fibrosis.
    Authors: Zakaria E, El-Maraghy N, Ahmed A, Ali A, El-Bassossy H
    Naunyn Schmiedebergs Arch Pharmacol, 0;390(6):621-631.  0
  13. Combinatorial effects of PARP inhibitor PJ34 and histone deacetylase inhibitor vorinostat on leukemia cell lines.
    Authors: Jasek E, Gajda M, Lis G, Jasinska M, Litwin J
    Anticancer Res, 0;34(4):1849-56.  0
  14. New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair.
    Authors: Jelinic P, Levine D
    Mol Cancer Ther, 0;13(6):1645-54.  0
  15. Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors.
    Authors: Thurber G, Reiner T, Yang K, Kohler R, Weissleder R
    Mol Cancer Ther, 0;13(4):986-95.  0
  16. Poly(ADP-ribose) polymerase 1 (PARP-1) binds to 8-oxoguanine-DNA glycosylase (OGG1).
    Authors: Noren Hooten N, Kompaniez K, Barnes J, Lohani A, Evans M
    J Biol Chem, 0;286(52):44679-90.  0
  17. Poly(ADP-ribose) polymerase 1 promotes oxidative-stress-induced liver cell death via suppressing farnesoid X receptor alpha.
    Authors: Wang C, Zhang F, Wang L, Zhang Y, Li X, Huang K, Du M, Liu F, Huang S, Guan Y, Huang D, Huang K
    Mol Cell Biol, 0;33(22):4492-503.  0
  18. Nitric oxide modulates hypoxia-inducible factor-1 and poly(ADP-ribose) polymerase-1 cross talk in response to hypobaric hypoxia.
    Authors: Martinez-Romero R, Canuelo A, Siles E, Oliver F, Martinez-Lara E
    J Appl Physiol (1985), 0;112(5):816-23.  0
  19. Cannabinoid 1 receptor promotes cardiac dysfunction, oxidative stress, inflammation, and fibrosis in diabetic cardiomyopathy.
    Authors: Rajesh M, Batkai S, Kechrid M, Mukhopadhyay P, Lee W, Horvath B, Holovac E, Cinar R, Liaudet L, Mackie K, Hasko G, Pacher P
    Diabetes, 0;61(3):716-27.  0
  20. Disruption of Wnt/beta-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells.
    Authors: Kulak O, Chen H, Holohan B, Wu X, He H, Borek D, Otwinowski Z, Yamaguchi K, Garofalo L, Ma Z, Wright W, Chen C, Shay J, Zhang X, Lum L
    Mol Cell Biol, 0;35(14):2425-35.  0
  21. The PARP inhibitors, veliparib and olaparib, are effective chemopreventive agents for delaying mammary tumor development in BRCA1-deficient mice.
    Authors: To C, Kim E, Royce D, Williams C, Collins R, Risingsong R, Sporn M, Liby K
    Cancer Prev Res (Phila), 0;7(7):698-707.  0
  22. Targeting on poly(ADP-ribose) polymerase activity with DNA-damaging hybrid lactam-steroid alkylators in wild-type and BRCA1-mutated ovarian cancer cells.
    Authors: Trafalis D, Polonifi A, Dalezis P, Nikoleousakos N, Katsamakas S, Sarli V
    Chem Biol Drug Des, 0;90(5):854-866.  0
  23. Acyl-CoA-binding domain containing 3 modulates NAD+ metabolism through activating poly(ADP-ribose) polymerase 1.
    Authors: Chen Y, Bang S, Park S, Shi H, Kim S
    Biochem J, 0;469(2):189-98.  0

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