PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
PARP Enzyme High Specific Activity
R&D Systems | Catalog # 4668-100-01
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Key Product Details
Features
Purified from E. coli containing a recombinant plasmid harboring human PARP1 gene.
Key Benefits
- Identification of inhibitors of PARP1 activity Quantitation of DNA Damage Investigation of PARP1 inactivation during apoptosis Western Blot Analysis
Species
Human
Product Summary for PARP Enzyme High Specific Activity
Substrate Specificity
PARP1 uses NAD+ as a substrate to catalyze poly(ADP-ribosyl)ation of target proteins involved in chromatin structure and DNA metabolism. PARP1 is stimulated by DNA-strand breaks and capable of auto-ribosylation.
Concentration
10 units/µl
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Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at ≤ -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for PARP Enzyme High Specific Activity
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for PARP Enzyme High Specific Activity
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