PARP Universal Colorimetric Assay Kit

Catalog # Availability Size / Price Qty
4677-096-K
Product Details
Citations (18)
FAQs
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PARP Universal Colorimetric Assay Kit Summary

The PARP Universal Colorimetric Assay (Catalog # 4677-096-K) measures Poly (ADP-ribose) Polymerase (PARP) in cells and tissues by detecting the incorporation of biotinylated Poly (ADP-ribose) (PAR) onto histone proteins in a 96-stripwell microplate format. This assay is useful for testing whether DNA is damaged, if damaged DNA is from non-apoptotic cells, or the effectiveness of PARP inhibitors.

Features

  • Colorimetric, non-radioactive format
  • Amenable to high-throughput
  • Highly sensitive: capable of measuring 0.01 Units of PARP/well

Kit Contents

  • Histone-coated 96-stripwell Microplate
  • PARP Enzyme-High Specific Activity
  • PARP Buffer
  • PARP Cocktaila
  • 3-aminobenzamideb
  • Streptavidin-HRP & Diluent
  • TACS-Sapphire™
  • Activated DNA

Data Example

Inhibition of PARP Activity by 3-aminobenzamide

Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit; B). The standard curve is used to translate absorbance values to activity units of PARP.

 

aPARP Cocktail contains biotinylated NAD
b3-aminobenzamide is a PARP inhibitor

TACS-Sapphire is a trademark of Trevigen, Inc.

Product Datasheets

Preparation and Storage

Stability & Storage
The product  requires storage at both -20 to -70 °C and 2 - 8 °C.  Consult the product insert for specific storage temperatures. Do not use past expiration date.

Background: PARP

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name
Poly [ADP-ribose] Polymerase
Entrez Gene IDs
142 (Human); 11545 (Mouse)
Alternate Names
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1

Citations for PARP Universal Colorimetric Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. TRAIL induces necroptosis involving RIPK1/RIPK3-dependent PARP-1 activation.
    Authors: Jouan-Lanhouet S, Arshad M, Piquet-Pellorce C, Martin-Chouly C, Le Moigne-Muller G, Van Herreweghe F, Takahashi N, Sergent O, Lagadic-Gossmann D, Vandenabeele P, Samson M, Dimanche-Boitrel M
    Cell Death Differ, 2012;19(12):2003-14.  2012
  2. Histone H2AX is a critical factor for cellular protection against DNA alkylating agents.
    Authors: Meador JA, Zhao M, Su Y, Narayan G, Geard CR, Balajee AS
    Oncogene, 2008;27(43):5662-71.  2008
  3. PARP-1 inhibition prevents oxidative and nitrosative stress-induced endothelial cell death via transactivation of the VEGF receptor 2.
    Authors: Mathews MT, Berk BC
    Arterioscler. Thromb. Vasc. Biol., 2008;28(4):711-7.  2008
  4. Combinatorial effects of PARP inhibitor PJ34 and histone deacetylase inhibitor vorinostat on leukemia cell lines.
    Authors: Jasek E, Gajda M, Lis G, Jasinska M, Litwin J
    Anticancer Res, 0;34(4):1849-56.  0
  5. Acyl-CoA-binding domain containing 3 modulates NAD+ metabolism through activating poly(ADP-ribose) polymerase 1.
    Authors: Chen Y, Bang S, Park S, Shi H, Kim S
    Biochem J, 0;469(2):189-98.  0
  6. BCL2 suppresses PARP1 function and nonapoptotic cell death.
    Authors: Dutta C, Day T, Kopp N, van Bodegom D, Davids M, Ryan J, Bird L, Kommajosyula N, Weigert O, Yoda A, Fung H, Brown J, Shapiro G, Letai A, Weinstock D
    Cancer Res, 0;72(16):4193-203.  0
  7. PARP inhibition ameliorates nephropathy in an animal model of type 2 diabetes: focus on oxidative stress, inflammation, and fibrosis.
    Authors: Zakaria E, El-Maraghy N, Ahmed A, Ali A, El-Bassossy H
    Naunyn Schmiedebergs Arch Pharmacol, 0;390(6):621-631.  0
  8. Differential effects of poly(ADP-ribose) polymerase inhibition on DNA break repair in human cells are revealed with Epstein-Barr virus.
    Authors: Ma W, Halweg C, Menendez D, Resnick M
    Proc Natl Acad Sci U S A, 0;109(17):6590-5.  0
  9. New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair.
    Authors: Jelinic P, Levine D
    Mol Cancer Ther, 0;13(6):1645-54.  0
  10. Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors.
    Authors: Thurber G, Reiner T, Yang K, Kohler R, Weissleder R
    Mol Cancer Ther, 0;13(4):986-95.  0
  11. Poly(ADP-ribose) polymerase 1 promotes oxidative-stress-induced liver cell death via suppressing farnesoid X receptor alpha.
    Authors: Wang C, Zhang F, Wang L, Zhang Y, Li X, Huang K, Du M, Liu F, Huang S, Guan Y, Huang D, Huang K
    Mol Cell Biol, 0;33(22):4492-503.  0
  12. Poly(ADP-ribose) polymerase 1 (PARP-1) binds to 8-oxoguanine-DNA glycosylase (OGG1).
    Authors: Noren Hooten N, Kompaniez K, Barnes J, Lohani A, Evans M
    J Biol Chem, 0;286(52):44679-90.  0
  13. Nitric oxide modulates hypoxia-inducible factor-1 and poly(ADP-ribose) polymerase-1 cross talk in response to hypobaric hypoxia.
    Authors: Martinez-Romero R, Canuelo A, Siles E, Oliver F, Martinez-Lara E
    J Appl Physiol (1985), 0;112(5):816-23.  0
  14. Cannabinoid 1 receptor promotes cardiac dysfunction, oxidative stress, inflammation, and fibrosis in diabetic cardiomyopathy.
    Authors: Rajesh M, Batkai S, Kechrid M, Mukhopadhyay P, Lee W, Horvath B, Holovac E, Cinar R, Liaudet L, Mackie K, Hasko G, Pacher P
    Diabetes, 0;61(3):716-27.  0
  15. Disruption of Wnt/beta-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells.
    Authors: Kulak O, Chen H, Holohan B, Wu X, He H, Borek D, Otwinowski Z, Yamaguchi K, Garofalo L, Ma Z, Wright W, Chen C, Shay J, Zhang X, Lum L
    Mol Cell Biol, 0;35(14):2425-35.  0
  16. The PARP inhibitors, veliparib and olaparib, are effective chemopreventive agents for delaying mammary tumor development in BRCA1-deficient mice.
    Authors: To C, Kim E, Royce D, Williams C, Collins R, Risingsong R, Sporn M, Liby K
    Cancer Prev Res (Phila), 0;7(7):698-707.  0
  17. Targeting on poly(ADP-ribose) polymerase activity with DNA-damaging hybrid lactam-steroid alkylators in wild-type and BRCA1-mutated ovarian cancer cells.
    Authors: Trafalis D, Polonifi A, Dalezis P, Nikoleousakos N, Katsamakas S, Sarli V
    Chem Biol Drug Des, 0;90(5):854-866.  0
  18. Silencing of poly(ADP-ribose) polymerase-1 suppresses hyperstretch-induced expression of inflammatory cytokines in vitro.
    Authors: Wang J, Liu L, Xia Y, Wu D
    Acta Biochim Biophys Sin (Shanghai), 0;46(7):556-64.  0

FAQs

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Supplemental Apoptosis Reagents

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