PARP3 Antibody (HL1509) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25593

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1509 expressed in HEK293

Format

Azide and BSA Free
View all PARP3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP3. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PARP3 Antibody (HL1509) - Azide and BSA Free

PARP3 Antibody (HL1509) - Azide and BSA Free

Western Blot: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] -

Western Blot: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP3 antibody [HL1509] (NBP3-25593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
PARP3 Antibody (HL1509) - Azide and BSA Free

Immunohistochemistry-Paraffin: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] -

Immunohistochemistry-Paraffin: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] - PARP3 antibody [HL1509] detects PARP3 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PARP3 stained by PARP3 antibody [HL1509] (NBP3-25593) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PARP3 Antibody (HL1509) - Azide and BSA Free

Western Blot: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] -

Western Blot: PARP3 Antibody (HL1509) - Azide and BSA Free [NBP3-25593] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP3 antibody [HL1509] (NBP3-25593) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PARP3 Antibody (HL1509) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARP3

PARP3 is encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Protein mono-ADP-ribosyltransferase PARP3

Alternate Names

ADPRT-3, ADPRT3, ADPRTL3, ARTD3, hPARP-3, pADPRT-3, PARP-3

Gene Symbol

PARP3

Additional PARP3 Products

Product Documents for PARP3 Antibody (HL1509) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PARP3 Antibody (HL1509) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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