Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-52350

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # L114/81

Format

BSA Free
View all Parvalbumin Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-110 (full-length) of rat Parvalbumin (Uniprot#: P02625) produced recombinantly in E. Coli

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L114/81 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L114/81 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

Western Blot: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Western Blot: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Western blotting of rat whole brain homogenate with NBP3-52350 (10 µg/ml) and detected with anti-chicken-HRP. This antibody recognizes endogenous Parvalbumin in the rat whole brain homogenate at ~12 kDa.
Immunohistochemistry-Paraffin: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Immunohistochemistry-Paraffin: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with NBP3-52350 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the Purkinje cells and neurons of the molecular layer in the cerebellum. Magnification 100X.
Immunohistochemistry-Paraffin: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Immunohistochemistry-Paraffin: Parvalbumin Antibody (L114/81) - Chimeric - BSA Free [NBP3-52350]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with NBP3-52350 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the Purkinje cells and neurons of the molecular layer in the cerebellum. Magnification 400X.

Applications for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

Application
Recommended Usage

Immunohistochemistry

-Free Floating

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parvalbumin

Parvalbumin is encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Alternate Names

D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV

Gene Symbol

PVALB

Additional Parvalbumin Products

Product Documents for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Parvalbumin Antibody (L114/81) - Chimeric - BSA Free

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  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

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