Parvalbumin Antibody - BSA Free

Novus Biologicals | Catalog # NB120-11427

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Feline

Cited:

Human, Mouse, Rat, Feline, Primate, Primate - Callithrix jacchus (Common Marmoset)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Parvalbumin Antibody made from purified parvalbumin

Reactivity Notes

Use in Feline reported in scientific literature (PMID:10.1016/j.celrep.2021.109083). Mouse reactivity reported in scientific literature (PMID: 24065886). Mouse reactivity reported in scientific literature (PMID:33053349).

Localization

Cytoplasmic and Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Parvalbumin Antibody - BSA Free

Western Blot: Parvalbumin AntibodyBSA Free [NB120-11427]

Western Blot: Parvalbumin AntibodyBSA Free [NB120-11427]

Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.
Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.
Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody - BSA Free [NB120-11427]

Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.
Parvalbumin Antibody - BSA Free

Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] -

Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm).
Parvalbumin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] -

Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Parvalbumin Antibody - BSA Free

Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] -

Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Parvalbumin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 33515957)

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1 ug/ml

Immunoprecipitation

5 ug/ml

Western Blot

0.1 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parvalbumin

Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

Alternate Names

D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV

Entrez Gene IDs

5816 (Human); 25269 (Rat)

Gene Symbol

PVALB

UniProt

Additional Parvalbumin Products

Product Documents for Parvalbumin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Parvalbumin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Parvalbumin Antibody - BSA Free

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Protocols

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FAQs for Parvalbumin Antibody - BSA Free

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  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

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