PAX1 Antibody

Novus Biologicals | Catalog # NBP3-12330

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
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Product Specifications

Immunogen

Synthetic peptide corresponding to unique sequence within amino acid region 210-260 on human PAX1 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PAX1 Antibody

Western Blot: PAX1 Antibody [NBP3-12330]

Western Blot: PAX1 Antibody [NBP3-12330]

Western Blot: PAX1 Antibody [NBP3-12330] - A. PC-PAX1, B. MCF7 cells. 1:500 antibody dilution in DiluObuffer, apparent MW is 63 KDa.
Immunohistochemistry-Paraffin: PAX1 Antibody [NBP3-12330]

Immunohistochemistry-Paraffin: PAX1 Antibody [NBP3-12330]

Immunohistochemistry-Paraffin: PAX1 Antibody [NBP3-12330] - Rat tongue. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for PAX1 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: PAX1

Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Alternate Names

paired box 1, paired domain gene HuP48

Gene Symbol

PAX1

Additional PAX1 Products

Product Documents for PAX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PAX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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