Pax2 Antibody (JJ082-08)

Novus Biologicals | Catalog # NBP2-67849

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ082-08 expressed in HEK293
View all Pax2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Pax2 aa 161-357 / 417. (SwissProt: Q02962 Human; SwissProt: D4ACZ2 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pax2 Antibody (JJ082-08)

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of Pax2 on human kidney tissue lysates with Rabbit anti-Pax2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Pax2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Western blot analysis of Pax2 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat
Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded human kidney tissue using anti-Pax2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pax2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Pax2 Antibody (JJ082-08)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:400

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax2

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Paired Box Gene 2

Alternate Names

paired box 2, paired box gene 2, paired box homeotic gene 2, paired box protein Pax-2

Gene Symbol

PAX2

Additional Pax2 Products

Product Documents for Pax2 Antibody (JJ082-08)

Certificate of Analysis

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Product Specific Notices for Pax2 Antibody (JJ082-08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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