Pax3 Antibody (HL160) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13661

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL160 expressed in HEK293

Format

Azide and BSA Free
View all Pax3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to Coturnix coturnix Pax3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Pax3 Antibody (HL160) - Azide and BSA Free

Western Blot: Pax3 Antibody (HL160) [NBP3-13661]

Western Blot: Pax3 Antibody (HL160) [NBP3-13661]

Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Pax3 Antibody (HL160) [NBP3-13661]

Western Blot: Pax3 Antibody (HL160) [NBP3-13661]

Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Pax3 Antibody (HL160)

Western Blot: Pax3 Antibody (HL160) [NBP3-13661] -

Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Pax3 Antibody (HL160)

Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661] -

Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661] - Pax3 antibody [HL160] detects Pax3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: Pax3 stained by Pax3 antibody [HL160] (NBP3-13661) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Pax3 Antibody (HL160) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax3

PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Long Name

Paired Box Gene 3 [Waardenburg Syndrome 1]

Alternate Names

HUP2, WS1

Gene Symbol

PAX3

Additional Pax3 Products

Product Documents for Pax3 Antibody (HL160) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Pax3 Antibody (HL160) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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