Pax3 Antibody

Novus Biologicals | Catalog # NB100-1420

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pax3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence TTLAGAVPRMM-C corresponding to N-Terminus according to NP_039230.1, NP_852122.1, NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1.

Epitope

TTLAGAVPRMM

Localization

Nuclear

Specificity

This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2; NP_039230.1; NP_852122.1;.1 NP_852123.1; NP_852124.1; NP_852125.1 and NP_852126.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pax3 Antibody

Western Blot: Pax3 Antibody [NB100-1420]

Western Blot: Pax3 Antibody [NB100-1420]

Western Blot: Pax3 Antibody [NB100-1420] - Staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420]

Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420]

Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420] - Staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420]

Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420]

Immunohistochemistry-Paraffin: Pax3 Antibody [NB100-1420] - Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.

Applications for Pax3 Antibody

Application
Recommended Usage

Immunohistochemistry

3 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

detection limit 1:128000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 45 kDa band observed in lysates of human brain (cerebellum), duodenum, kidney and Skin, in rat duodenum and stomach lysates, in A431 and HeLa lysates, and in nuclear lysates of Jurkat (calculated MW of 45.2 kDa band according to NP_852125.1 and of 44.8 kDa band according to NP_852126.1). IHC-P: Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Data obtained from a previous batch (different goat). Use in immunoprecipitation reported in scientific literature (PMID: 16945326).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pax3

This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Long Name

Paired Box Gene 3 [Waardenburg Syndrome 1]

Alternate Names

HUP2, WS1

Entrez Gene IDs

5077 (Human); 18505 (Mouse); 114502 (Rat)

Gene Symbol

PAX3

UniProt

NP_000429

Additional Pax3 Products

Product Documents for Pax3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Pax3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax3 Antibody

There are currently no reviews for this product. Be the first to review Pax3 Antibody and earn rewards!

Have you used Pax3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies