Pax6 Antibody (PAX6/1166)
Novus Biologicals | Catalog # NBP2-44576
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PAX6/1166
Loading...
Product Specifications
Immunogen
Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)
Localization
Nuclear
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47915)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Pax6 Antibody (PAX6/1166)
![Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576]](https://resources.rndsystems.com/images/products/Pax6-Antibody-PAX6-1166-Immunohistochemistry-Paraffin-NBP2-44576-img0001.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576]")
Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576]
Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) [NBP2-44576] - Human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (PAX6/1166).![Knockout Validated: Pax6 Antibody (PAX6/1166) [NBP2-44576]](https://resources.rndsystems.com/images/products/Pax6-Antibody-PAX6-1166-Knockout-Validated-NBP2-44576-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (PAX6/1166) [NBP2-44576]")
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (PAX6/1166) [NBP2-44576]
Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (PAX6/1166) [NBP2-44576] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44576) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).Applications for Pax6 Antibody (PAX6/1166)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Pax6
Long Name
Paired Box Gene 6
Alternate Names
keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6
Gene Symbol
PAX6
UniProt
Additional Pax6 Products
Product Documents for Pax6 Antibody (PAX6/1166)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pax6 Antibody (PAX6/1166)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Pax6 Antibody (PAX6/1166)
There are currently no reviews for this product. Be the first to review Pax6 Antibody (PAX6/1166) and earn rewards!
Have you used Pax6 Antibody (PAX6/1166)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Pax6 Antibody (PAX6/1166)
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
A:
By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.
