Pax6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89100

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYW

Marker

Neural Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pax6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP1-89100]

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP1-89100]

Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer
Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.

Applications for Pax6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Gene Symbol

PAX6

UniProt

P26367

Additional Pax6 Products

Product Documents for Pax6 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pax6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pax6 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pax6 Antibody - BSA Free

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  • Q: What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

    A:

    By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

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