Pax7 Antibody (PAX7/497) [Allophycocyanin]

Novus Biologicals | Catalog # NBP2-34706APC

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAX7/497
View all Pax7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)

Localization

Nuclear

Marker

Rhabdomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for Pax7 Antibody (PAX7/497) [Allophycocyanin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-34706APC in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pax7

PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Long Name

Paired Box Gene 7

Alternate Names

HUP1, RMS2

Gene Symbol

PAX7

Additional Pax7 Products

Product Documents for Pax7 Antibody (PAX7/497) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Pax7 Antibody (PAX7/497) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax7 Antibody (PAX7/497) [Allophycocyanin] (1)

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  • Pax7 Antibody (PAX7/497) [Allophycocyanin]
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse skeletal muscle
    Species: Mouse
    Verified Customer | Posted 04/18/2017
    Sample Information Cell Lines Tested: mouse skeletal muscle-derived primary cell population Species: mouse Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 Fixation Solution and Conditions: 4% formaldehyde treatment for 20min at RT Blocking Solution: no-treatment because of using fluorescence conjugated antibody in this experiment Time and Temperature: Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Primary Antibody Diluent: 0.5% tritonX-100 in PBS Dilutions Tested: 1:50 Time and Temperature: overnight at 4℃ Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times Detection System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 633nm Emission Filter: 691nm
    Pax7 Antibody (PAX7/497) [Allophycocyanin] NBP2-34706APC

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