PAX8 Antibody (1F8-3A8)

Novus Biologicals | Catalog # NBP2-29903

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey, Primate

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F8-3A8
View all PAX8 Primary Antibodies »

Product Specifications

Immunogen

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

Reactivity Notes

Mouse reactivity reported in a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PAX8 Antibody (1F8-3A8)

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.
Immunocytochemistry/ Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.
Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.
Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.
Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903]

Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).

Applications for PAX8 Antibody (1F8-3A8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:20 - 1:200

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:500 - 1:2000
Application Notes
WB: Detects a prominent approx. 48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8. IHC reported in a review from a verified customer.

Reviewed Applications

Read 1 review rated 5 using NBP2-29903 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAX8

PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. Pax8 is expressed in the developing and adult thyroid and kidney. In the thyroid, PAX8 regulates thyroglobulin, thyroidperoxidase,and NIS (sodium/iodide symporter) expression. Mutations in PAX8 have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas.

Alternate Names

paired box 8, paired box gene 8, paired box protein Pax-8, paired domain gene 8

Entrez Gene IDs

7849 (Human)

Gene Symbol

PAX8

UniProt

Q06710 (Human)

Additional PAX8 Products

Product Documents for PAX8 Antibody (1F8-3A8)

Certificate of Analysis

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Product Specific Notices for PAX8 Antibody (1F8-3A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PAX8 Antibody (1F8-3A8)

Customer Reviews for PAX8 Antibody (1F8-3A8) (1)

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Customer Images

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  • PAX8 Antibody (1F8-3A8)
    Name: Feng Li
    Application: Immunocytochemistry
    Sample Tested: thyroid tumor tissue
    Species: Mouse
    Verified Customer | Posted 05/30/2017
    Pax8 staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100
    PAX8 Antibody (1F8-3A8) NBP2-29903

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