Paxillin Antibody (S04-3C3)
Novus Biologicals | Catalog # NBP3-20046
Recombinant Monoclonal Antibody
![Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046]](https://resources.rndsystems.com/images/products/Paxillin-Antibody-S04-3C3-Western-Blot-NBP3-20046-img0003.jpg "Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S04-3C3 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human Paxillin (Uniprot # P49023)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Paxillin Antibody (S04-3C3)
Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046]
Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046] - Western blot detection of Paxillin in C6 cell lysates using NBP3-20046 (1:1000 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.![Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046]](https://resources.rndsystems.com/images/products/Paxillin-Antibody-S04-3C3-Immunohistochemistry-Paraffin-NBP3-20046-img0002.jpg "Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046]")
Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046]
Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunohistochemistry of Paxillin in paraffin-embedded Human breast cancer tissue using NBP3-20046 at dilution 1/50
Immunocytochemistry/Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046] -
Immunocytochemistry/Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunofluorescence of Paxillin (green) in hela using NBP3-20046 at dilution 1/5, and DAPI (blue)Applications for Paxillin Antibody (S04-3C3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/50
Immunohistochemistry
1/20-1/100
Immunohistochemistry-Paraffin
1:20-1:100
Western Blot
1/1000-1/5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Paxillin
Alternate Names
PXN
Gene Symbol
PXN
Additional Paxillin Products
Product Documents for Paxillin Antibody (S04-3C3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Paxillin Antibody (S04-3C3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
VEGF - VEGF R2 Signaling Pathways
