PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

Novus Biologicals | Catalog # NBP2-42207

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S159-5

Format

BSA Free
View all PCDHGA1 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Rat.

Localization

Cell Membrane

Specificity

Detects approx 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

Western Blot: PCDHGA1 Antibody (S159-5)Pan Specific [NBP2-42207]

Western Blot: PCDHGA1 Antibody (S159-5)Pan Specific [NBP2-42207]

Western Blot: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa PCDHGA1 protein using Mouse Anti-PCDHGA1 Monoclonal Antibody, Clone S159-5 (NBP2-42207). Load: 10 ug. Primary Antibody: Mouse Anti-PCDHGA1 Monoclonal Antibody (NBP2-42207) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
Immunocytochemistry/ Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207]

Immunocytochemistry/ Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207]

Immunocytochemistry/Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody, Clone S159-5 (NBP2-42207). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody (NBP2-42207) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PCDHGA1 (pan) Antibody (D) Composite.
Western Blot: PCDHGA1 Antibody (S159-5)Pan Specific [NBP2-42207]

Western Blot: PCDHGA1 Antibody (S159-5)Pan Specific [NBP2-42207]

Western Blot: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - analysis of Rat Brain Membrane showing detection of 100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 ug. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: 100 kDa.
Immunocytochemistry/ Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207]

Immunocytochemistry/ Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207]

Immunocytochemistry/Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody, Clone S159-5 (NBP2-42207). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody (NBP2-42207) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) PCDHGA1 (pan) Antibody. (D) Composite.

Applications for PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Protocadherin Gamma (pan) Antibody was sufficient for detection of Protocadherin gamma (pan) in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCDHGA1

The PCDHGA1 gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked onchromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism maybe involved in their regulat

Alternate Names

MGC138287, MGC138289, PCDH-GAMMA-A1, protocadherin gamma subfamily A, 1, protocadherin gamma-A1

Gene Symbol

PCDHGA1

Additional PCDHGA1 Products

Product Documents for PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

Certificate of Analysis

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Product Specific Notices for PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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