PCNA Antibody (7H4F8) - BSA Free

Novus Biologicals | Catalog # NBP2-37414

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H4F8

Format

BSA Free
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PCNA (AA: 53-196) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCNA Antibody (7H4F8) - BSA Free

Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.
Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA antibody. Antibody dilution 1:500. Image from verified customer review.
Flow Cytometry: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Flow Cytometry: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Flow Cytometry: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).
Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against human PCNA recombinant protein. (Expected MW is 41.2 kDa)
Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8)BSA Free [NBP2-37414]

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of colon cancer tissues using PCNA mouse mAb with DAB staining.
ELISA: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

ELISA: PCNA Antibody (7H4F8) - BSA Free [NBP2-37414]

ELISA: PCNA Antibody (7H4F8) [NBP2-37414] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PCNA Antibody (7H4F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 5 using NBP2-37414 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (7H4F8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PCNA Antibody (7H4F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PCNA Antibody (7H4F8) - BSA Free (1)

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  • PCNA Antibody (7H4F8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Cervical cancer tissue
    Species: Human
    Verified Customer | Posted 01/23/2022
    Cervical cancer
    Antibody dilution 1:500
    PCNA Antibody (7H4F8) - BSA Free NBP2-37414

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Protocols

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