PCNA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89503

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCA

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-89503]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-89503]

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).
PCNA Antibody - BSA Free Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Analysis in human lymph node and liver tissues using NBP1-89503 antibody. Corresponding PCNA RNA-seq data are presented for the same tissues.
PCNA Antibody - BSA Free Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Staining of human lymph node shows strong nuclear positivity in germinal center cells.
PCNA Antibody - BSA Free Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Staining of human liver shows no positivity in hepatocytes as expexted.
PCNA Antibody - BSA Free Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Staining of human colon shows strong nuclear positivity in glandular cells.
PCNA Antibody - BSA Free Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Immunohistochemistry-Paraffin: PCNA Antibody - BSA Free [NBP1-89503]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
PCNA Antibody - BSA Free Western Blot: PCNA Antibody - BSA Free [NBP1-89503]

Western Blot: PCNA Antibody - BSA Free [NBP1-89503]

Analysis in human cell line MOLT-4.
PCNA Antibody - BSA Free Western Blot: PCNA Antibody - BSA Free [NBP1-89503]

Western Blot: PCNA Antibody - BSA Free [NBP1-89503]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for PCNA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PCNA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody - BSA Free

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Protocols

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FAQs

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