PCYT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83952

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCYT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCYT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human gastrointestinal, kidney, liver and testis using Anti-PCYT2 antibody NBP1-83952 (A) shows similar protein distribution across tissues to independent antibody NBP1-83951 (B).
Western Blot: PCYT2 Antibody [NBP1-83952]

Western Blot: PCYT2 Antibody [NBP1-83952]

Western Blot: PCYT2 Antibody [NBP1-83952] - Analysis using Anti-PCYT2 antibody NBP1-83952 (A) shows similar pattern to independent antibody NBP1-83951 (B).
Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952]

Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83952] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
PCYT2 Antibody - BSA Free Western Blot: PCYT2 Antibody - BSA Free [NBP1-83952]

Western Blot: PCYT2 Antibody - BSA Free [NBP1-83952]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells))
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells))

Applications for PCYT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCYT2

ECT (ethanolamine phosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis. PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression. Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE. The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn). Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer's disease.

Long Name

Ethanolamine-phosphate cytidylyltransferase

Alternate Names

EC 2.7.7.14

Gene Symbol

PCYT2

Additional PCYT2 Products

Product Documents for PCYT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PCYT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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