PD-1 Antibody (4F12) - BSA Free

Novus Biologicals | Catalog # NBP1-04328

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Canine

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F12

Format

BSA Free
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Product Specifications

Immunogen

Recombinant human PD-1 (21-167aa) purified from E. coli

Reactivity Notes

Use in Canine reported in scientific literature (PMID:34923192).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PD-1 Antibody (4F12) - BSA Free

Western Blot: PD-1 Antibody (4F12)BSA Free [NBP1-04328]

Western Blot: PD-1 Antibody (4F12)BSA Free [NBP1-04328]

Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate
Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]

Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]

Immunocytochemistry/Immunofluorescence: PD-1 Antibody (4F12) [NBP1-04328] - HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]

Flow Cytometry: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]

Flow Cytometry: PD-1 Antibody (4F12) [NBP1-04328] - Flow cytometry analysis of PDCD1 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Applications for PD-1 Antibody (4F12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

2-5ug

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-1

PDCD1 is an immunoreceptor that belongs to the immunoglobulin (Ig) superfamily, also known as Programmed Cell Death 1. It is expressed on activated T cell, B cells, myeloid cells and a subset of thymocytes. PDCD1 interacts with expressed PDL1 and PDL2 to inhibit the proliferation and activation of T cells and B cells. Both PDL1 and PDL2 are expressed in a wide variety of normal tissues including heart, lung, placenta, spleen and kidney. PDCD1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

UniProt

AAH74740 (Human)

Additional PD-1 Products

Product Documents for PD-1 Antibody (4F12) - BSA Free

Certificate of Analysis

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Product Specific Notices for PD-1 Antibody (4F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PD-1 Antibody (4F12) - BSA Free

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Protocols

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