PD-1 Antibody (CL3624) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44083

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3624

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q15116, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PD-1 Antibody (CL3624) - Azide and BSA Free

PD-1 Antibody (CL3624) - Azide and BSA Free Western Blot: PD-1 Antibody [NBP3-44083]

Western Blot: PD-1 Antibody [NBP3-44083]

Lane 1: Marker [kDa] 250)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells)
PD-1 Antibody (CL3624) - Azide and BSA Free Immunohistochemistry-Paraffin: PD-1 Antibody [NBP3-44083]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP3-44083]

Staining of human liver shows absence of immunoreactivity (negative control).
PD-1 Antibody (CL3624) - Azide and BSA Free Immunohistochemistry-Paraffin: PD-1 Antibody [NBP3-44083]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP3-44083]

Staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, mainly in the reaction centrum.

Applications for PD-1 Antibody (CL3624) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-1

PD1 is an inhibitory cell membrane surface receptor from the immunoglobulin superfamily. Involved in the regulation of T cell function during immunity and tolerance, PD1 inhibits T cell effector functions upon ligand binding. The protein is expressed in pro-B-cells and is thought to play a role in their differentiation as well. Studies have also suggested that this gene product may also contribute to the prevention of autoimmune diseases.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for PD-1 Antibody (CL3624) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PD-1 Antibody (CL3624) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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