PD-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88104

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PD-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PD-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining in human lymph node and liver tissues. Corresponding PDCD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]

Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
PD-1 Antibody - BSA Free Western Blot: PD-1 Antibody - BSA Free [NBP1-88104]

Western Blot: PD-1 Antibody - BSA Free [NBP1-88104]

Analysis in control (vector only transfected HEK293T lysate) and pDCD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for PD-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: NK cell lysate at 1 mg/ml, NSCLC lysate at 1 mg/ml as samples; separated by size; antibody dilution of 1:50; observed molecular weight was 116 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-1

PD1 is an inhibitory cell membrane surface receptor from the immunoglobulin superfamily. Involved in the regulation of T cell function during immunity and tolerance, PD1 inhibits T cell effector functions upon ligand binding. The protein is expressed in pro-B-cells and is thought to play a role in their differentiation as well. Studies have also suggested that this gene product may also contribute to the prevention of autoimmune diseases.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for PD-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PD-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PD-1 Antibody - BSA Free

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Protocols

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FAQs

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