Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # Z64P2D3*H4

Format

BSA Free
View all PD-L2/B7-DC Primary Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide KAVFFKRHN

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free [NBP2-52408]

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free [NBP2-52408]

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3-H4) [NBP2-52408] - Staining was performed on formalin-fixed, paraffin-embedded human testis seminoma tissue sections using anti-PDL2 (soluble form) [Z64P2D3*H4]. Cytoplasmic staining was observed.

Applications for PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100 - 1:2000
Application Notes
For IHC, perform antigen retrieval step: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Positive control(s): IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-L2/B7-DC

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. B7-DC (CD273) has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. B7-DC (CD273) has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Gene Symbol

PDCD1LG2

Additional PD-L2/B7-DC Products

Product Documents for PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

Certificate of Analysis

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Product Specific Notices for PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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