PDCD4 Antibody (3A8V10)
Novus Biologicals | Catalog # NBP3-16744
Recombinant Monoclonal Antibody
![Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744]](https://resources.rndsystems.com/images/products/PDCD4-Antibody-3A8V10-Western-Blot-NBP3-16744-img0003.jpg "Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3A8V10 expressed in HEK293
Loading...
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 370-469 of human PDCD4 (Q53EL6). MVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDCD4 Antibody (3A8V10)
Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744]
Western Blot: PDCD4 Antibody (3A8V10) [NBP3-16744] - Western blot analysis of extracts of various cell lines, using PDCD4 Rabbit mAb (NBP3-16744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.![Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]](https://resources.rndsystems.com/images/products/PDCD4-Antibody-3A8V10-Immunohistochemistry-Paraffin-NBP3-16744-img0002.jpg "Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]")
Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]
Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744] - Immunohistochemistry of paraffin-embedded human placenta using PDCD4 Rabbit mAb (NBP3-16744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]](https://resources.rndsystems.com/images/products/PDCD4-Antibody-3A8V10-Immunohistochemistry-Paraffin-NBP3-16744-img0001.jpg "Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]")
Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744]
Immunohistochemistry-Paraffin: PDCD4 Antibody (3A8V10) [NBP3-16744] - Immunohistochemistry of paraffin-embedded rat lung using PDCD4 Rabbit mAb (NBP3-16744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for PDCD4 Antibody (3A8V10)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PDCD4
Long Name
Programmed Cell Death 4
Alternate Names
H731, Protein 197/15a
Gene Symbol
PDCD4
Additional PDCD4 Products
Product Documents for PDCD4 Antibody (3A8V10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PDCD4 Antibody (3A8V10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for PDCD4 Antibody (3A8V10)
There are currently no reviews for this product. Be the first to review PDCD4 Antibody (3A8V10) and earn rewards!
Have you used PDCD4 Antibody (3A8V10)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
