PDCD6 Antibody (A6A7-R)

Western Blot: PDCD6 Antibody (A6A7-R) [NBP3-32728]

Western Blot: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Western blot analysis of PDCD6 on different lysates with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/2,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32728) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728]

Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: PDCD6 Antibody (A6A7-R) [NBP3-32728]

Flow Cytometry: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Flow cytometric analysis of HeLa cells labeling PDCD6.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32728, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).