PDE1B Antibody (5C4A3) - BSA Free

Novus Biologicals | Catalog # NBP2-37404

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C4A3

Format

BSA Free
View all PDE1B Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PDE1B (AA: 370-536) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE1B Antibody (5C4A3) - BSA Free

Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3) [NBP2-37404] - Western blot analysis using PDE1B mouse mAb against PC-12 (1) cell lysate.
Immunohistochemistry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Immunohistochemistry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Immunohistochemistry: PDE1B Antibody (5C4A3) [NBP2-37404] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PDE1B mouse mAb with DAB staining.
Flow Cytometry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Flow Cytometry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Flow Cytometry: PDE1B Antibody (5C4A3) [NBP2-37404] - Flow cytometric analysis of A549 cells using PDE1B mouse mAb (green) and negative control (red).
Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3) [NBP2-37404] - Western blot analysis using PDE1B mAb against human PDE1B recombinant protein. (Expected MW is 44.4 kDa)
Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3)BSA Free [NBP2-37404]

Western Blot: PDE1B Antibody (5C4A3) [NBP2-37404] - Western blot analysis using PDE1B mAb against HEK293 (1) and PDE1B (AA: 370-536)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Immunohistochemistry: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

Immunohistochemistry: PDE1B Antibody (5C4A3) [NBP2-37404] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PDE1B mouse mAb with DAB staining.
ELISA: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

ELISA: PDE1B Antibody (5C4A3) - BSA Free [NBP2-37404]

ELISA: PDE1B Antibody (5C4A3) [NBP2-37404] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PDE1B Antibody (5C4A3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE1B

PDE1B, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids.

Long Name

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Alternate Names

63 kDa Cam-PDE, Cam-PDE 1B, PDES1B

Gene Symbol

PDE1B

Additional PDE1B Products

Product Documents for PDE1B Antibody (5C4A3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PDE1B Antibody (5C4A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PDE1B Antibody (5C4A3) - BSA Free

There are currently no reviews for this product. Be the first to review PDE1B Antibody (5C4A3) - BSA Free and earn rewards!

Have you used PDE1B Antibody (5C4A3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies