PDE1B Antibody (OTI6E4)

Novus Biologicals | Catalog # NBP2-01063

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI6E4
View all PDE1B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE1B Antibody (OTI6E4)

Western Blot: PDE1B Antibody (OTI6E4) [NBP2-01063]

Western Blot: PDE1B Antibody (OTI6E4) [NBP2-01063]

Western Blot: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
Immunocytochemistry/Immunofluorescence: PDE1B Antibody (6E4) [NBP2-01063] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.
Immunohistochemistry-Paraffin: PDE1B Antibody (OTI6E4) [NBP2-01063]

Immunohistochemistry-Paraffin: PDE1B Antibody (OTI6E4) [NBP2-01063]

Immunohistochemistry-Paraffin: PDE1B Antibody (6E4) [NBP2-01063] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody.
Flow Cytometry: PDE1B Antibody (OTI6E4) [NBP2-01063]

Flow Cytometry: PDE1B Antibody (OTI6E4) [NBP2-01063]

Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody, and then analyzed by flow cytometry.
Flow Cytometry: PDE1B Antibody (OTI6E4) [NBP2-01063]

Flow Cytometry: PDE1B Antibody (OTI6E4) [NBP2-01063]

Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - Analysis of Jurkat cells, using anti-PDE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for PDE1B Antibody (OTI6E4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE1B

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].

Long Name

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Alternate Names

63 kDa Cam-PDE, Cam-PDE 1B, PDES1B

Entrez Gene IDs

5153 (Human)

Gene Symbol

PDE1B

Additional PDE1B Products

Product Documents for PDE1B Antibody (OTI6E4)

Certificate of Analysis

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Product Specific Notices for PDE1B Antibody (OTI6E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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