PDE5A Antibody (9H5) - Azide and BSA Free

Novus Biologicals | Catalog # H00008654-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Plant

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 9H5

Format

Azide and BSA Free
View all PDE5A Primary Antibodies »

Product Specifications

Immunogen

PDE5A (NP_001074, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP

Specificity

PDE5A - phosphodiesterase 5A, cGMP-specific

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDE5A Antibody (9H5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PDE5A Antibody (9H5) [H00008654-M01]

Immunocytochemistry/ Immunofluorescence: PDE5A Antibody (9H5) [H00008654-M01]

Immunocytochemistry/Immunofluorescence: PDE5A Antibody (9H5) [H00008654-M01] - Analysis of monoclonal antibody to PDE5A on HeLa cell. Antibody concentration 20 ug/ml.
Sandwich ELISA: PDE5A Antibody (9H5) [H00008654-M01] - Detection limit for recombinant GST tagged PDE5A is 1 ng/ml as a capture antibody.

Applications for PDE5A Antibody (9H5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDE5A

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Long Name

cGMP-specific 3',5'-cyclic phosphodiesterase

Alternate Names

CGB-PDE, PDE5

Entrez Gene IDs

8654 (Human)

Gene Symbol

PDE5A

OMIM

603310 (Human)

UniProt

NP_001074 (Human)

Additional PDE5A Products

Product Documents for PDE5A Antibody (9H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PDE5A Antibody (9H5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDE5A Antibody (9H5) - Azide and BSA Free

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Protocols

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FAQs

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