PDE5A Antibody

Novus Biologicals | Catalog # NB300-655

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic cyclic peptide (common to all PDE5A variants) (Rat).

Localization

Located mostly to soluble cellular fractions, and sometimes to particulate cellular fractions.

Specificity

This detects 99 and 89 kDa PDE5A proteins in various tissues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE5A Antibody

Western Blot: PDE5A Antibody [NB300-655]

Western Blot: PDE5A Antibody [NB300-655]

Western Blot: PDE5A Antibody [NB300-655] - 50 ug of rat lung Homogentate.
Immunocytochemistry/ Immunofluorescence: PDE5A Antibody [NB300-655]

Immunocytochemistry/ Immunofluorescence: PDE5A Antibody [NB300-655]

Immunocytochemistry/Immunofluorescence: PDE5A Antibody [NB300-655] - Cells expressing PDE5 with antibody.
Western Blot: PDE5A Antibody [NB300-655]

Western Blot: PDE5A Antibody [NB300-655]

Western Blot: PDE5A Antibody [NB300-655] - Antibody dilution 1:500 in diluObuffer

Applications for PDE5A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:250

Western Blot

1:500
Application Notes
This antibody can be used in Immunoprecipitation and Western Blot. In Western Blot a 89 and 99 kDa bands are seen (predicted MW is 105kDa). Recommended Starting Dilutions*: IP: 1/250 WB: 1/500 Not tested in other applications. *Optimal dilutions should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE5A

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (PDE5A) family is comprised of 2 genes (PDE5A and PDE5B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. al., 2002; Murthy 2001). PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP-kinases (McAllister et al., 93).

Long Name

cGMP-specific 3',5'-cyclic phosphodiesterase

Alternate Names

CGB-PDE, PDE5

Entrez Gene IDs

8654 (Human); 242202 (Mouse); 171115 (Rat)

Gene Symbol

PDE5A

Additional PDE5A Products

Product Documents for PDE5A Antibody

Certificate of Analysis

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Product Specific Notices for PDE5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PDE5A Antibody

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  • Q: What buffer is NB300-655 supplied in?

    A: NB300-655 is supplied in a proprietary antibody stabilization buffer. The constituents are PBS, Tris-glycine, stabilizing proteins, glycerol and other cryoprotectants, but we cannot disclose the exact concentrations. If you need to conjugate this antibody or couple it, we can supply it in primary amine-free buffer.

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