PDE6D Antibody (HL1359)

Novus Biologicals | Catalog # NBP3-48654

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Feline, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1359
View all PDE6D Primary Antibodies »

Product Specifications

Immunogen

Full length human PDE6D Recombinant protein

Reactivity Notes

Japanese Medaka (100%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE6D Antibody (HL1359)

PDE6D Antibody (HL1359)

Immunocytochemistry/ Immunofluorescence: PDE6D Antibody (HL1359) [NBP3-48654] -

PDE6D antibody [HL1359] detects PDE6D protein at nucleus by immunofluorescent analysis.
Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:500.
Blue: Fluoroshield with DAPI.
PDE6D Antibody (HL1359)

Immunohistochemistry-Paraffin: PDE6D Antibody (HL1359) [NBP3-48654] -

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PDE6D Antibody (HL1359)

Immunohistochemistry-Paraffin: PDE6D Antibody (HL1359) [NBP3-48654] -

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Whole Japanese medaka extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PDE6D Antibody (HL1359)

Immunohistochemistry-Paraffin: PDE6D Antibody (HL1359) [NBP3-48654] -

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PDE6D Antibody (HL1359)

Immunohistochemistry-Paraffin: PDE6D Antibody (HL1359) [NBP3-48654] -

PDE6D antibody [HL1359] detects PDE6D protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PDE6D stained by PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PDE6D Antibody (HL1359)

Western Blot: PDE6D Antibody (HL1359) [NBP3-48654] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [HL1359] (NBP3-48654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PDE6D Antibody (HL1359)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE6D

PDE6D is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha® (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha®/alpha® homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

Alternate Names

GMP-PDE delta, PDED, Protein p17

Gene Symbol

PDE6D

Additional PDE6D Products

Product Documents for PDE6D Antibody (HL1359)

Certificate of Analysis

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Product Specific Notices for PDE6D Antibody (HL1359)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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