PDE6D Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32730

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Xenopus

Predicted:

Bovine (99%), Canine (98%), Rhesus Macaque (99%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDE6D Primary Antibodies »

Product Specifications

Immunogen

Full length human PDE6D Recombinant protein.

Reactivity Notes

Rat (85%). Xenopus reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE6D Antibody - BSA Free

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730] - SAS xenograft, using PDE6D antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PDE6D Antibody [NBP1-32730]

Western Blot: PDE6D Antibody [NBP1-32730]

Western Blot: PDE6D Antibody [NBP1-32730] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
PDE6D Antibody

Immunocytochemistry/Immunofluorescence: PDE6D Antibody [NBP1-32730] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE6D stained by PDE6D antibody [N1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10μm

Immunocytochemistry/Immunofluorescence: PDE6D Antibody [NBP1-32730] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE6D stained by PDE6D antibody [N1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um
PDE6D Antibody

Western Blot: PDE6D Antibody [NBP1-32730] -

Western Blot: PDE6D Antibody [NBP1-32730] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] (NBP1-32730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PDE6D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Reviewed Applications

Read 1 review rated 4 using NBP1-32730 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDE6D

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alpha beta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDE alpha beta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

Alternate Names

GMP-PDE delta, PDED, Protein p17

Gene Symbol

PDE6D

Additional PDE6D Products

Product Documents for PDE6D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PDE6D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for PDE6D Antibody - BSA Free

Customer Reviews for PDE6D Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used PDE6D Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • PDE6D Antibody
    Name: Nycole Maza
    Application: Western Blot
    Sample Tested: X. laevis whole retina lysate: 50ug/well
    Species: Xenopus laevis
    Verified Customer | Posted 08/03/2018
    Western Blot using NBP1-32730 anti-PDE6D (1:500 dilution) on X. laevis retinal lysate (50ug/well). Faint band detected.
    Used at 1:500 dilution. Detected with Mouse anti-rabbit secondary antibody.
    PDE6D Antibody - BSA Free NBP1-32730

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies